Qualitative and Quantitative Analysis of Total Chlorogenic Acids in Green Coffee Beans

The burden of chronic diseases related to diet and nutrition is rapidly increasing worldwide. This due to rapid change in life style, and presents greatest public heath burden either in terms of direct cost to society and government, or in terms of disability adjusted life years (DALYs). This includes diabetes, obesity, cardiovascular diseases and cancer. These diseases can be controlled by use of chlorogenic acids (CGAs) which have many biological properties including; antioxidant, antiviral, anti-carcinogenic, free radical scavenging and removal of toxic metals through chelation. In this study CGAs were extracted and characterized from green coffee beans (GCBs), Coffee canephora (Robusta) for their use as dietary supplement. This is done through quantitative and qualitative analysis of CGAs in green coffee beans harvested from Machakos County, Kathiani constituency, lower Kaewa ward. In order to achieve this GCBs were milled to 8-9 mesh size to expose CGAs for easy extraction, extracted in 80% aqueous methanol at 65-70 °C, defatted using hexane, decaffeinated using dichloromethane and extracted using ethyl acetate. The GCBs were investigated and found to assay 8.82% CGAs after analysis by High Performance Liquid Chromatography (HPLC). While the extracted product assayed 81.40±9.60% CGAs. This project is quite significant for its contribution to public health sector through prevention and control of chronic diseases. It is also significant to the agricultural sector one of the main economic hub in Kenya by providing market for Robusta coffee which does fetch poor prices in instant coffee manufacturing industries due to high CGAs content, labor sector and the economy at large. 

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