Extraction of Compounds from the Leaves of Azadirachta Indica and Anti-Microbial Analysis

Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each part of the plant has some medicinal properties. Phytochemical studies were carried out on Azadirachta indica leaves. Fresh leaves of the plant were collected from Lamu, Kenya shade- dried and ground into powder using a blender.  The crude extracts were obtained via soxhlet extraction with hexane and dichloromethane. Concentration was done in vacuo on a rotary evaporator. Thin layer chromatography analysis of the crude extract was done. Isolation of the compounds was achieved via column chromatography which was packed with silica gel and 100% n-Hexane. The bioassay test for anti-microbial activity using crude extract showed the inhibition zones against Staphylococcus aureus and Escherichia coli. In both cases, the inhibition zones were significantly enhanced by the pure compounds obtained from column chromatography. Spot tests were carried out and the results obtained showed the presence of flavones, flavonoids flavonols, chalcones and sterols.

 

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