Phytochemical Analysis of Raspberry Roots

Raspberry belongs to Rosaceae family of plants. Rubus idaeus commonly known as red raspberry contains phytochemicals which have medicinal properties. Among phytochemicals of interest found in the plant roots are namely anthocyanin; flavonoids; phenolic compounds and benzoquinones. Previously, these labile compounds and other antioxidants products have been studied using GC-MS, IR, UV and H-NMR which have given a lot of information about the products. In this research, the root sample was extracted by cold percolation using Dichloromethane/Methanol (1:1 v/v). The sample was concentrated by rotary evaporator to give a concentrated extract. The crude extract was subjected to column chromatography upon which five pure compounds were isolated.

The isolated compounds were then analyzed with IR and GC-MS for structure elucidation. The major compounds identified by the GCMS include Urs-12-en-24-oicacid,3-oxo-,methyl ester,(+)-, and Urs-12-en-24-oic acid among others such as 3- oxo- methyl ester,  Octamethyl, 14b- Octadecahydro-2H-picen-3-one, Pentadecanoic acid, 14-methyl-, methyl ester ,  9,12Octadecadienoyl Α-Tocopherol / Vitamin E and Urs-12-ene.

The crude extract and the five fractions obtained from the column were tasted for their antimicrobial activity against Escherichia coli and showed good antimicrobial activity.

Keywords: Rubus idaeus, phytochemicals, Antimicrobial activity.

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