Isolation and Characterization of Arial Surface Compounds of Tarchonanthus camphorates

 

 

 

Phytochemistry literature reports on T. camphoratus point to the presence of surface exudates on the aerial parts of the plant because of the observed methylated flavonoids. However no study expressly carried out with this realization has been reported; such an understanding usually leads to isolation of many more compounds from such species.

 

For a more in depth view of the species, plant leaves were collected from Naivasha area. The leaves were washed by dipping into ethyl acetate for 15 seconds to remove the surface exudates only and to avoid extraction of the internal tissue components including chlorophyll.

 

The extract was then concentrated into a gummy residue using a rotary evaporator. The residue was then loaded on a silica gel column and separated, the separation being followed by using analytical thin layer chromatography (TLC) plates. Fractions obtained were combined on the basis of TLC profiles. Fractions were further resolved using preparative TLC and crystallization methods to obtain pure compounds.

 

Structures of the following four compounds isolated were elucidated using spectroscopic evidence of 1H and 13C NMR and found to be flavonoids.

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